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KMID : 0545119910010020142
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Journal of Microbiology and Biotechnology
1991 Volume.1 No. 2 p.142 ~ p.149
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Suspension Culture of Gardenia jasminoides Ellis Cell for Production of Yellow Pigment
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Kim, Sang Hwa
Park, Young Goo/Lee, Yong Hyun
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Abstract
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Gardenia callus was Induced in MS medium containing 10μM of 2,4 diphenoxy acetic acid (2,4-D), 1μM kinetin, and 3% sucrose in the dark. B_5 medium was identified to be the most adequate medium for cell growth. Indole-3-acetic acid (IAA) was better growth regulator than 2,4-D not only for cell growth but slso for carotenoid production. Ligt also played a critical role on synthesis of carotenoid. Gardenia cells grown in B_5 medium could utilize a polysaccharide, soluble starch, as a carbon source. The cell growth was stimulated in B_5 medium fortified with 0.2% yeast extract. The optimum pH for cell growth was 5.7. High density cultures can be maintained by increasing inoculum size and medium concentration accordingly. Specific growth rate and mass doubling time were 0.095 day^-1 and 7.3 days, respectively. The cell immobilized in alginate tends to formulate more enlarged vacuoles containing yellow pigment compared with those of suspended cell. Carotenold content of immobilized cell was about 264.4㎍/g fresh weight (F.W.) corresponding twice of the content of suspended cell (112.08㎍/g F.W.). The color of gardenia cell was shifted from yellow to red when carbohydrase-secreting fungus, Trichoderma reesei, was co-cultivated with gardenia cells.
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